The application of biosecurity in the aquaculture industry is seen as very important as one of the determinants of sustainable production. This application protects aquaculture from high mortality rates and low growth rates due to infection from pathogenic microorganisms and the introduction of exotic pathogenic that act as disease carriers. However, many traditional fish farmers have not implemented biosecurity due to limited capital. This paper objective is to assess the costs of biosecurity application in different aquaculture system in the traditional fish farm. This study collected data from previous literature and continued by cost simulation. Then we linked the results with the possible impact on health. The results prove that some practices in applying biosecurity in traditional fish farms have limited benefits due to an imbalance between the operating costs and substantial investments compare to fish productivity. Limited protection from biosecurity application caused a threat to fish and human health. This study concluded that biosecurity in traditional fish farms had limited ability to protect fish and human health