ABSTRACT DNA methylation (DNAm) plays a crucial role in a number of complex diseases.
However, the reliability of DNAm levels measured using Illumina arrays varies across
different probes. Previous research primarily assessed probe reliability by comparing
duplicate samples between the 450k-450k or 450k-EPIC platforms, with limited
investigations on Illumina EPIC v1. 0 arrays. We conducted a comprehensive assessment of
the EPIC v1. 0 array probe reliability using 69 blood DNA samples, each measured twice …

In their recent work, Zhang et al. comprehensively evaluated the reliability of DNA
methylation probes on the Illumina MethylationEPIC v1. 0 BeadChip microarray using
intraclass correlation coefficients (ICCs) calculated based on duplicated samples [1]. Using
the observed ICCs, they classified probes into the four reliability categories
'Excellent,''Good,''Fair,'and 'Poor,'and shared their results with the readership, creating a
valuable resource for future studies. Their downstream analyses demonstrated that higher …