Cryo-EM structure of Escherichia coli σ70 RNA polymerase and promoter DNA complex revealed a role of σ non-conserved region during the open complex formation
First step of gene expression is transcribing the genetic information stored in DNA to RNA by
the transcription machinery including RNA polymerase (RNAP). In Escherichia coli, a
primary σ 70 factor forms the RNAP holoenzyme to express housekeeping genes. The σ 70
contains a large insertion between the conserved regions 1.2 and 2.1, the σ non-conserved
region (σ NCR), but its function remains to be elucidated. In this study, we determined the
cryo-EM structures of the E. coli RNAP σ 70 holoenzyme and its complex with promoter DNA …
the transcription machinery including RNA polymerase (RNAP). In Escherichia coli, a
primary σ 70 factor forms the RNAP holoenzyme to express housekeeping genes. The σ 70
contains a large insertion between the conserved regions 1.2 and 2.1, the σ non-conserved
region (σ NCR), but its function remains to be elucidated. In this study, we determined the
cryo-EM structures of the E. coli RNAP σ 70 holoenzyme and its complex with promoter DNA …