Cy3 Photoprotection Mediated by Ni2+ for Extended Single-Molecule Imaging: Old Tricks for New Techniques
The photostability of reporter fluorophores in single-molecule fluorescence imaging is of
paramount importance, as it dictates the amount of relevant information that may be
acquired before photobleaching occurs. Quenchers of triplet excited states are thus required
to minimize blinking and sensitization of singlet oxygen. Through a combination of single-
molecule studies and ensemble mechanistic studies including laser flash photolysis and
time-resolved fluorescence, we demonstrate herein that Ni2+ provides a much desired …
paramount importance, as it dictates the amount of relevant information that may be
acquired before photobleaching occurs. Quenchers of triplet excited states are thus required
to minimize blinking and sensitization of singlet oxygen. Through a combination of single-
molecule studies and ensemble mechanistic studies including laser flash photolysis and
time-resolved fluorescence, we demonstrate herein that Ni2+ provides a much desired …
[引用][C] Cy3 Photoprotection Mediated by Ni2+ for Extended Single-Molecule Imaging: Old Tricks for New Techniques
G Viktorija, L Richard, C Gonzalo - 2015
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