Cyanobacterial small chlorophyll-binding protein ScpD (HliB) is located on the periphery of photosystem II in the vicinity of PsbH and CP47 subunits
Cyanobacteria contain several genes coding for small one-helix proteins called SCPs or
HLIPs with significant sequence similarity to chlorophyll a/b-binding proteins. To localize
one of these proteins, ScpD, in the cells of the cyanobacterium Synechocystis sp. PCC 6803,
we constructed several mutants in which ScpD was expressed as a His-tagged protein
(ScpDHis). Using two-dimensional native-SDS electrophoresis of thylakoid membranes or
isolated Photosystem II (PSII), we determined that after high-light treatment most of the …
HLIPs with significant sequence similarity to chlorophyll a/b-binding proteins. To localize
one of these proteins, ScpD, in the cells of the cyanobacterium Synechocystis sp. PCC 6803,
we constructed several mutants in which ScpD was expressed as a His-tagged protein
(ScpDHis). Using two-dimensional native-SDS electrophoresis of thylakoid membranes or
isolated Photosystem II (PSII), we determined that after high-light treatment most of the …
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