The increasing industrialisation and urbanisation have deteriorated the quality and quantity of water bodies, harming the surrounding flora and fauna. Therefore, in our studies, we have chosen the HEK293 cell line to examine further the level of wastewater toxicity to which living beings are exposed. The water samples were collected from various sites around the Agra Canal in the Faridabad region of Haryana. Furthermore, cytotoxicity and genotoxicity confirmation of wastewater samples were done by MTT and comet assay, respectively. The water quality of the Agra canal is heavily influenced by agricultural, domestic, and industrial waste, which may affect the genetic material of species exposed to contaminated water and the sustainability of the local environment. As a result, continuous environmental monitoring and proper policy formulation are required to minimise the adverse effects of pollutants in waste, which would further enrich India’s preparation to take India a step ahead, and that could be the best possible way to commemorate India’s 75th year of Independence with the Azadi Ka Amrit Mahotsav.