DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
Nature methods, 2013•nature.com
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography
solvents lead to a strong enhancement of electrospray ionization of peptides, improving the
sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can
be easily implemented on any LC-MS/MS system without modification to hardware or
software and at no additional cost.
solvents lead to a strong enhancement of electrospray ionization of peptides, improving the
sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can
be easily implemented on any LC-MS/MS system without modification to hardware or
software and at no additional cost.
Abstract
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
nature.com
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