DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices
Background MeCP2 and MBD2 are members of a family of proteins that possess a domain
that selectively binds 5-methylcytosine in a CpG context. Members of the family interact with
other proteins to modulate DNA packing. Stretching of DNA–protein complexes in
nanofluidic channels with a cross-section of a few persistence lengths allows us to probe the
degree of compaction by proteins. Results We demonstrate DNA compaction by MeCP2
while MBD2 does not affect DNA configuration. By using atomic force microscopy (AFM), we …
that selectively binds 5-methylcytosine in a CpG context. Members of the family interact with
other proteins to modulate DNA packing. Stretching of DNA–protein complexes in
nanofluidic channels with a cross-section of a few persistence lengths allows us to probe the
degree of compaction by proteins. Results We demonstrate DNA compaction by MeCP2
while MBD2 does not affect DNA configuration. By using atomic force microscopy (AFM), we …
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