DNA profiling of azoospermic semen samples from vasectomized males by using Y-PLEX™ 6 amplification kit
JG Shewale, E Schneida, SD Sinha… - Journal of forensic …, 2003 - astm.org
JG Shewale, E Schneida, SD Sinha, SC Sikka
Journal of forensic sciences, 2003•astm.orgPost-vasectomized azoospermic semen samples (N= 6) were analyzed for short tandem
repeats (STR) on the Y-chromosome by using Y-PLEX™ 6 and the 310 Genetic Analyzer.
We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This
variation was attributed to the number of epithelial and/or white blood cells that are present
in these azoospermic samples. DNA profiles of these vasectomized males were obtained for
all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 …
repeats (STR) on the Y-chromosome by using Y-PLEX™ 6 and the 310 Genetic Analyzer.
We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This
variation was attributed to the number of epithelial and/or white blood cells that are present
in these azoospermic samples. DNA profiles of these vasectomized males were obtained for
all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 …
Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX™6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX™6.
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