Summary Double homeobox 4 (DUX4) is expressed at the early pre-implantation stage in
human embryos. Here we show that induced human DUX4 expression substantially alters
the chromatin accessibility of non-coding DNA and activates thousands of newly identified
transcribed enhancer-like regions, preferentially located within ERVL-MaLR repeat
elements. CRISPR activation of transcribed enhancers by C-terminal DUX4 motifs results in
the increased expression of target embryonic genome activation (EGA) genes ZSCAN4 and …