Deep learning enables fast, gentle STED microscopy
Communications biology, 2023•nature.com
STED microscopy is widely used to image subcellular structures with super-resolution. Here,
we report that restoring STED images with deep learning can mitigate photobleaching and
photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our
method allows for efficient and robust restoration of noisy 2D and 3D STED images with
multiple targets and facilitates long-term imaging of mitochondrial dynamics.
we report that restoring STED images with deep learning can mitigate photobleaching and
photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our
method allows for efficient and robust restoration of noisy 2D and 3D STED images with
multiple targets and facilitates long-term imaging of mitochondrial dynamics.
Abstract
STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that restoring STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics.
nature.com
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