A Model of the Quaternary Structure of Enolases, Based on Structural and Evolutionary Analysis of the Octameric Enolase from Bacillus subtilis

CK Brown, PL Kuhlman, S Mattingly, K Slates… - Journal of protein …, 1998 - Springer
… There are therefore six possible subunit combinations for this enzyme, which include three
… We also are indebted to Andy Denham for help in the design and contruction of the growth …

Assembly of minicellulosomes on the surface of Bacillus subtilis

TD Anderson, SA Robson, XW Jiang… - Applied and …, 2011 - Am Soc Microbiol
… strains of Bacillus subtilis that display one or more cellulase enzymes. … enzyme is fused
to the appropriate dockerin module. The enzymatic properties of a number of purified designer

Selection of effective and highly thermostable Bacillus subtilis lipase A template as an industrial biocatalyst-A modern computational approach

B Senthilkumar, D Meshachpaul, R Sethumadhavan… - Frontiers in biology, 2015 - Springer
… for designing thermostable … enzymes secreted by Bacillus subtilis are lipase LipA (EC
3.1.1.3), esterase LipB (EC 3.1.1.1) and phospholipase. The atomic mass for Bacillus subtilis

A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis

V Jayaraman, DJ Lee, N Elad, S Vimer… - Nature chemical …, 2022 - nature.com
… Multi-enzyme assemblies composed of metabolic enzymes catalyzing … multi-enzyme
complex from Bacillus subtilis composed of two enzymes catalyzing opposite (‘counter-enzymes’) …

Enhancing catalytic efficiency of Bacillus subtilis laccase BsCotA through active site pocket design

Y Hou, L Zhao, C Yue, J Yang, Y Zheng… - Applied Microbiology …, 2024 - Springer
… Slight improvement in T 50 was noted in the F-09 and F-28 design, with 72.53 C and
71.87 C, respectively. This complements the fact that our designs do not alter the enzyme’s …

Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

FC Wu, CW Chang, IL Shih - SpringerPlus, 2013 - Springer
… the factorial design, Box–Wilson central composite design (… the optimum, an experimental
design with more than two levels … a full 2 4 factorial design augmented by eight axial points (or …

Enhancing the secretion of a feruloyl esterase in Bacillus subtilis by signal peptide screening and rational design

P Liu, J Guo, L Miao, H Liu - Protein Expression and Purification, 2022 - Elsevier
… This study provides a promising candidate for ferulic acid production and demonstrates
that the secretion of a heterologous enzyme from B. subtilis can be cumulatively improved by …

Secretory expression, characterization and docking study of glucose-tolerant β-glucosidase from B. subtilis

S Chamoli, P Kumar, NK Navani, AK Verma - International Journal of …, 2016 - Elsevier
… Thus, we report the BGL from B. subtilis is a novel efficient promising enzyme for industrial
… insights of BGL from Bacillus and believes that novel enzymatic properties reported in this …

Optimizing of pharmaceutical active compounds biodegradability in secondary effluents by β-lactamase from Bacillus subtilis using central composite design

A Al-Gheethi, E Noman, RMSR Mohamed… - Journal of hazardous …, 2019 - Elsevier
… effluents by using B. subtilis 2012WTNC as a function of β-lactamase was optimized using
response surface methodology (RSM) designed by central composite design (CCD). Four …

Structural and kinetic analysis of Bacillus subtilis N-acetylglucosaminidase reveals a unique Asp-His dyad mechanism

S Litzinger, S Fischer, P Polzer, K Diederichs… - Journal of Biological …, 2010 - ASBMB
… N-Acetylglucosaminyl enzyme intermediate accumulated in the H234G mutant … 234 of the
dyad of BsNagZ and, in contrast to the latter, protrudes from a second domain of the enzyme