Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure
L Baert, CE Wobus, E Van Coillie… - Applied and …, 2008 - Am Soc Microbiol
L Baert, CE Wobus, E Van Coillie, LB Thackray, J Debevere, M Uyttendaele
Applied and environmental microbiology, 2008•Am Soc MicrobiolThe correlation between the detection of murine norovirus 1 RNA by real-time reverse
transcription-PCR and the infectivity by plaque assay before and after heat exposure (80° C)
was examined. No correlation was found in the current study. Moreover, heat inactivation
had a much stronger detrimental effect on virus infectivity than on the integrity of the viral
genome.
transcription-PCR and the infectivity by plaque assay before and after heat exposure (80° C)
was examined. No correlation was found in the current study. Moreover, heat inactivation
had a much stronger detrimental effect on virus infectivity than on the integrity of the viral
genome.
Abstract
The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.
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