Superior mutant rodent tuber plant (Typhoniumflagelliforme) is a medicinal herb of Indonesia, which immensely useful for anticancer activity. Some studies reported that the leaves and the tubers, conventionally parts of rodent tuber plant, showed various anticancer, antimicrobial, and antioxidant potential. This study aim is to determine the bioactive compounds of superior mutant rodent tuber plants through n-hexane and ethyl acetate fractions using GC-MS for the analysis. Phytochemical characterization of the superior mutant rodent tuber plant extracts was detected by qualitative analysis. A total of 20 bioactive compounds were obtained in an n-hexane fraction. A total of 4 bioactive compounds were identified in ethyl acetate fraction. The GC-MS analysis showed the presence of the major compound in ethyl acetate and n-hexane fraction were 9, 11-octadecadienoic acid, methyl ester (55.37%), 9-octadecenoic acid (Z)-, methyl ester (26.77%), hexadecanoic acid, methyl ester (2.97%), 9, 12-octadecadienoyl chloride (2.47%), humulene (1.11%), octahydronapthalene (0.97%), alloaromadendrene oxide-(2)(0.85%), pentadecanoic acid, methyl ester (0.80%), methyl tetradecanoate (0.79%), and eucalyptol (0.69%). Most of the identified compounds in the n-hexane and ethyl acetate fractions exhibit following biology activity, such as anticancer, antioxidant, anti-androgenic, antimicrobial, antifungal, and anti-inflammatory. This study provides an overview of the chemical compounds and their beneficial impact on developing drugs from n-hexane and ethyl acetate fraction of the superior mutant rodent tuber plant.