Determination of tramadol in human plasma and urine samples using liquid phase microextraction with back extraction combined with high performance liquid …
Liquid phase microextraction by back extraction (LPME–BE) combined with high
performance liquid chromatography (HPLC)-fluorescence detection was developed for the
determination of tramadol in human plasma. Tramadol was extracted from 2mL of basic
sample solution (donor phase) with pH 11.5 through a micro liter-size organic solvent phase
(100μL n-octane) for 25min and finally into a 3.5 μL acidic aqueous acceptor microdrop with
pH 2.5 suspended in the organic phase from the tip of a HPLC microsyringe needle for …
performance liquid chromatography (HPLC)-fluorescence detection was developed for the
determination of tramadol in human plasma. Tramadol was extracted from 2mL of basic
sample solution (donor phase) with pH 11.5 through a micro liter-size organic solvent phase
(100μL n-octane) for 25min and finally into a 3.5 μL acidic aqueous acceptor microdrop with
pH 2.5 suspended in the organic phase from the tip of a HPLC microsyringe needle for …
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