Isoflavones are widely used as an alternative treatment to hormone replacement therapy
and also for prevention of several chronic diseases, including cancers. Genistein, daidzein
and glycitein are the most abundant isoflavone aglycones found in soy extracts, where they
also occur as glycosides. This paper describes the development and validation of an
isocratic reversed-phase HPLC (RP-HPLC) method for the analysis of isoflavone aglycones,
released after acid hydrolysis of soy dry extracts, used as pharmaceutical raw material. The …