Development of 4-cell mouse embryos after re-vitrification
This paper reports studies on the effects of re-vitrification by the CPS (Closed Pulled Straw)
method on the development of 4-cell stage mouse embryos. The procedure involved
culturing 2-cell mouse embryos in G-1 medium until the 4-cell stage followed by the division
of the normal 4-cell stage embryos into a control group (non-vitrified) and two experimental
subgroups (vitrified and re-vitrified). Embryos in the vitrified subgroup were cryopreserved by
the CPS vitrification method. In the second experimental subgroup (re-vitrified), embryos that …
method on the development of 4-cell stage mouse embryos. The procedure involved
culturing 2-cell mouse embryos in G-1 medium until the 4-cell stage followed by the division
of the normal 4-cell stage embryos into a control group (non-vitrified) and two experimental
subgroups (vitrified and re-vitrified). Embryos in the vitrified subgroup were cryopreserved by
the CPS vitrification method. In the second experimental subgroup (re-vitrified), embryos that …
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