Development of indirect competitive ELISA for quantification of mitragynine in Kratom (Mitragyna speciosa (Roxb.) Korth.)

S Limsuwanchote, J Wungsintaweekul… - Forensic Science …, 2014 - Elsevier
Monoclonal antibody (MAb) against mitragynine (MG), an analgesic alkaloid from Kratom
leaves (Mitragyna speciosa), was produced. MG was coupled to carrier proteins employing
either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), a
zero-length cross linker or a 5-carbon length glutaraldehyde cross linker. To confirm the
immunogenicity, the hapten numbers were determined using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Preparation of the …
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