Although clinical trials of stimulation of angiogenesis by transfection of angiogenic growth
factors using naked plasmid DNA or adenoviral vector have been successful, there are still
unresolved problems for human gene therapy such as low transfection efficiency and safety.
From this viewpoint, it is necessary to develop safe and efficient novel nonviral gene transfer
methods. As therapeutic ultrasound induces cell membrane permeabilization, ultrasound
irradiation might increase the transfection efficiency of naked plasmid DNA into skeletal …

Development of safe and efficient Nove nonviral gene transfer using ultrasound:
enhancement of transfecton efficy of naked plasmid DNA in skeletal MUScle | CiNii Research
… CiNii Books … Development of safe and efficient Nove nonviral gene transfer using
ultrasound: enhancement of transfecton efficy of naked plasmid DNA in skeletal MUScle …