… We reasoned that if both samples could be run on the same gel, then the … matched sets of
fluorescent dyes that allows one to compare two or three protein samples on a single gel. In the …

… The dyes are mass matched and add 672.85 Da and 684.86 Da (monoisotopic mass addition
for Cy3 is 672.2982 and for Cy5 is 684.2981), respectively to the protein. Spectral qualities …

… two samples with two different dyes, running them on the same 2-D gel, post-run fluorescence
imaging of the gel into two … a matched-pair of amine reactive fluorescent dyes (Fig. 1) for …

… by acting as a loading control, but also aids in spot matching across gels, … dye-specific and
can be explained at least partly by differences in the background signals of the different dyes, …

… the use of fluorescence two-dimensional differential in-gel elec… Paired NK1R2/2 and NK1R1/1
samples were labeled with … dyes and electrophoresed on the same two-dimensional gels. …

… variation and performance of the different cyanine dye combinations and to evaluate the
quantitative … For this study and in our current work, we use the approach of matching differences …

… use of cyanine dyes for prelabelling and gel electrophoresis and report comparative results
demonstrating that these cyanine dyes … and matching were also performed using the single …

… for gel matching. These approaches have had limited success because the sources of … the
steps we use in performing 2D DIGE experiments. We typically use a two-dye approach with …

… Difference gel electrophoresis (DIGE), in which two protein samples are separately labeled
with different fluorescent dyes and then co-electrophoresed … design matched fluorescent dyes …

… dyes before isoelectric focusing, enabling the labeling of 2–3 samples with different dyes and
electrophoresis of … is to match the protein spots across all the gels and images. After image …