Direct immobilization of polyphenol oxidases on Celite 545 from ammonium sulphate fractionated proteins of potato (Solanum tuberosum)
In this study an effort has been made to use ammonium sulphate fractionated potato proteins
for the direct immobilization of polyphenol oxidase on an adsorbent, Celite 545. The yield of
Celite immobilized PPO activity was maximum in the buffer of pH, 7.0 and the support
retained 240units of PPO per gram. The immobilized potato PPO preparation exhibited
effectiveness factor 'η'as 0.71. Immobilized enzyme preparation was significantly more
resistant to the denaturation induced by pH, temperature, urea, detergents; SDS, Triton X …
for the direct immobilization of polyphenol oxidase on an adsorbent, Celite 545. The yield of
Celite immobilized PPO activity was maximum in the buffer of pH, 7.0 and the support
retained 240units of PPO per gram. The immobilized potato PPO preparation exhibited
effectiveness factor 'η'as 0.71. Immobilized enzyme preparation was significantly more
resistant to the denaturation induced by pH, temperature, urea, detergents; SDS, Triton X …
以上显示的是最相近的搜索结果。 查看全部搜索结果