In 2017, cases of generalized tuberculosis (TB) were identified in a cow and a bull‐camel, slaughtered at the Gusau abattoir in Zamfara State Nigeria. The objective of this study was to identify the cause of the gross pathology on the account of disseminated lesions widely distributed in different body parts of the animals. Tissue samples were analysed using Ziehl–Neelsen (ZN) stain and region of difference (RD) deletion typing. Results obtained by laboratory investigations revealed infection with acid‐fast tubercle bacilli in affected tissues from the cow and the dromedary bull‐camel. This study presents the first molecular evidence of Mycobacterium bovis infection in a Nigerian dromedary camel, demonstrated the ease of identification of the acid‐fast bacilli using molecular method which improves diagnosis and can aid epidemiological studies.