Dynamic assay for profiling anti-SARS-CoV-2 antibodies and their ACE2/Spike RBD neutralization capacity

T Phelan, J Dunne, N Conlon, CN Cheallaigh… - Viruses, 2021 - mdpi.com
T Phelan, J Dunne, N Conlon, CN Cheallaigh, WM Abbott, R Faba-Rodriguez, F Amanat…
Viruses, 2021mdpi.com
Serological assays have been widely employed during the coronavirus disease 2019
(COVID-19) pandemic to measure antibody responses to severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) and to track seroconversion in populations. However, currently
available assays do not allow determination of neutralization capacity within the assay
protocol. Furthermore, commercial serology assays have a high buy-in cost that is
inaccessible for many research groups. We have replicated the serological enzyme-linked …
Serological assays have been widely employed during the coronavirus disease 2019 (COVID-19) pandemic to measure antibody responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to track seroconversion in populations. However, currently available assays do not allow determination of neutralization capacity within the assay protocol. Furthermore, commercial serology assays have a high buy-in cost that is inaccessible for many research groups. We have replicated the serological enzyme-linked immunosorbent assay for the detection of SARS-CoV-2 antibody isotypes, developed at the Icahn School of Medicine at Mount Sinai, New York. Additionally, we have modified the protocol to include a neutralization assay with only a minor modification to this protocol. We used this assay to screen local COVID-19 patient sera (n = 91) and pre-COVID-19 control sera (n = 103), and obtained approximate parity with approved commercial anti-nucleoprotein-based assays with these sera. Furthermore, data from our neutralization assay closely aligns with that generated using a spike-based pseudovirus infection model when a subset of patient sera was analyzed.
MDPI
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