Segmental bone loss due to trauma, infection, and tumor resection and even non-union results in the vast demand for replacement and restoration of the function of the lost bone. The objective of present study was to evaluate effect of Calcium Phosphate granules on the healing of femoral bone defects in rabbits. Ten New Zealand white rabbits were used, of male gender, aged around 24 week and weighing 3.3-3.5 kg. Then animals were divided into two groups (control and experiment), with five rabbits in each. At the end of the experiment histopathologic and Histomorphometric parameters were evaluated. Histopathologic and Histomorphometric evaluations in present study showed that calcium phosphate granules improve osteogenesis in the defect area but more other researches are need yet.