[PDF][PDF] Effect of N-butanol Extract of Ficus glumosa Leaves on CCl4-induced Hepatotoxicity and Oxidative Stress in Rats

MS Abu, OE Yakubu, C Imo, RE Yohanna… - … Science Journal of …, 2021 - researchgate.net
MS Abu, OE Yakubu, C Imo, RE Yohanna, O Okpe, RL Mashi
Open Science Journal of Bioscience and Bioengineering, 2021researchgate.net
Liver plays crucial roles in transforming and clearing toxic chemical substances ingested
into the body and thus needs to be investigated for wide spectrum of protective and
ameliorative therapeutic agents that are readily available and affordable. In this study,
ameliorative effects of fractionated crude methanol extract of Ficus glumosa leaves against
hepatotoxicity and oxidative stress generated by CCl4 in albino rats was evaluated. The
crude methanol extract of Ficus glumosa leaves was re-dissolved in 300 ml of distilled water …
Abstract
Liver plays crucial roles in transforming and clearing toxic chemical substances ingested into the body and thus needs to be investigated for wide spectrum of protective and ameliorative therapeutic agents that are readily available and affordable. In this study, ameliorative effects of fractionated crude methanol extract of Ficus glumosa leaves against hepatotoxicity and oxidative stress generated by CCl4 in albino rats was evaluated. The crude methanol extract of Ficus glumosa leaves was re-dissolved in 300 ml of distilled water and repeatedly partitioned in a separating funnel with 400 ml of n-hexane for three times with vigorous shaking. At each portioning, the mixture was allowed to stand for 30 minutes to separate into distinct layers of hexane and aqueous. The n-hexane fraction was then collected and concentrated using water bath. The aqueous layer was then repeatedly partitioned with 400 ml of ethylacetate for three times to obtain ethylacetate fraction. The aqueous layer from the above was then saturated with distilled water and repeatedly partitioned with 400 ml of n-butanol for three times after which the n-butanol fraction was obtained. A total of 35 albino rats were used. The rats were distributed into 7 groups of 5 animals each. Rats were induced once weekly with 1ml/kg/body weight of 50% Carbon tetrachloride (CCl4) in olive oil and treated daily with three different graded doses (100 mg/kg/bw, 300 mg/kg/bw and 500 mg/kg/bw) of n-butanol fraction of Ficus glumosa leaves extract and 100 mg/kg/bw silymarin for 21 days. Consequently, the administration of n-butanol fraction significantly (p< 0.05) reduced liver marker enzymes (Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP)), bilirubin, malondialdehyde (MDA) but significantly (p< 0.05) increase serum proteins, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels in the treated rats as compared to the untreated rats. The results suggest that n-butanol fraction of methanol extract of Ficus glumosa leaves could possibly ameliorate hepatotoxicity and oxidative stress induced by CCl4 in the albino rats.
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