This investigation was carried out during Kharif, 2015 and Rabi, 2016 at Department of Seed Science and Technology and Department of Agricultural Microbiology and Bio-energy, College of Agriculture, PJTSAU, Rajendranagar, Hyderabad, Telangana. To study the viability and shelf life of biological agents on the seed quality and storability, pigeon pea (LRG-41) seed was coated with biological organisms like Trichoderma viride, Rhizobium and PSB by using two adjuvants like bio friendly polymer and sugar syrup and the treated seed was kept for six months storage. The viability and shelf life of biological agents were tested through Colony Forming Units (CFU) count throughout the storage period by monthly interval and seed quality and storability parameters were tested by bi-monthly interval. In combinational treatments of Trichoderma viride with bio fertilizers, Trichoderma viride showed lower count (18.4 x 105 cfu g-1) but Rhizobium showed higher count (16.8 x 105 cfu g-1) in one combination. Whereas, in another combination, Trichoderma viride showed still lower count (13.0 x 105 cfu g-1) and PSB also showed lower count (12.4 x 105 cfu g-1). The adjuvants like bio friendly polymer and sugar syrup, between these biological seed coating with bio-friendly polymer showed more viability and long shelf life (CFU) which were sufficient for sufficient colonization to give protection to the young seedlings against soil borne pathogens (compared to farmer practice of sugar syrup).