The present study was used to examine the effect of ambient temperature on the day of
slaughter of buffaloes on oocyte cleavage and subsequent embryo development following in
vitro fertilization (IVF)/chemical activation (parthenogenesis). A total of 601 oocytes were
collected from buffaloes, which were sacrificed when the ambient temperature was> 40 or≤
40° C and the collected oocytes were matured in vitro. During each experiment about half of
the matured oocytes were used for IVF whereas the remaining oocytes were subjected to …