Phytosome is a novel drug delivery system, containing phytoconstituents and phospholipids complex that enhance oral absorption dan bioavailability of phytoconstituents or herbal extracts. In this study, we investigate the effects of mole ratio luteolin to phosphatidylcholine on physicochemical properties of luteolin-loaded phytosome. Luteolin-loaded phytosomes (LLPs) were prepared in five different ratios by thin film hydration method. The resulting LLPs were then characterized using particle size analyzer (PSA), transmission electron microscopy (TEM), and fourier transforms infrared spectroscopy (FTIR). The LLPs that produced with mole ratio 1: 1 luteolin to phosphatidylcholine gave better physicochemical properties such as particle size, PDI values and zeta potential as well as entrapment efficiency and luteolin content, compared to those of other ratios. Hence, the optimum ratio of phytoconstituents to phospholipids in phytosome preparation is 1: 1.