The aim of this research to study the effects of oral intake of pineapple leaves extract (PLE) at three dosages (250, 500 and 750 mg/kg b. wt.) for 4weeksonhypercholesterolemic rats on serum levels of total cholesterol (TC), triglycerides (TG), atherogenic index (AI), lipoprotein fractions, Glucose and insulin concentration were performed. Thirty male Wister Albino rats were distributed into five groups as follows: group1: negative control group, group 2: positive control (hypercholesterolemia rats) group, groups 3, 4 and 5 fed on High cholesterol and high fat diet (HCFD) and treated with orally doses of PLE of 250, 500 and 750mg/kg b. wt., respectively. Results indicated that, the total content of phenolic compound of pineapple leaves extract was 22.28 mg/100 ml. The phenolic compounds were separated based on their functional groups into 25 fractions and identified 24 compounds from them by HPLC. The oral intake of PLE to hypercholesterolemia rats for 4 weeks significantly decreased serum levels of TC, TG, low density lipoproteins cholesterol (LDL-c), very low density lipoproteins cholesterol (VLDL-c), atherogenic index (AI) and Glucose concentration when compared to the control positive group. Levels of high density lipoprotein cholesterol (HDL-c) and insulin concentration were significantly increased as compared to the control positive group. There is a potent antiatherogenic and antioxidant effect of PLE on hypercholesterolemic rats.