O. rhinoceros horn beetle (Coleoptera: Scarabaeidae) is the main pest attacking oil palm crops in Indonesia, especially in palm oil rejuvenation areas. The study was conducted from April to August 2016 in the laboratory of the Faculty of Agriculture, Al-Azhar University, Medan. The materials used in this research are horn beetle pest larvae (O. rhinoceros) originating from PT. Socfin Indonesia, Trichoderma sp fungi originating from the Food Crops and Horticultural Fields of Medan and the B. bassiana fungi are derived from the Plant Seed Plant Protection Center (PBPPTP), aqua pro injection, Tween 80, rice and 96% alcohol. This research uses Completely Randomized Design (RAL) Non Factorial consisting of 13 treatments. The result of mortality of O. rhinoceros larvae on 1-14 DAA observation can be seen in appendix 2-43. Based on fingerprint analysis showed that the application of Trichoderma sp and B. basianna fungi on O. rhinoceros larvae had no significant effect on observation of 1 DAA to 9 DAA, but had a very significant effect on observation of 10 DAA to 14 DAA. The results of germination of Trichoderma sp and B. basianna fungus 4 hours after incubation period can be seen in appendix 61-66. Precentages mortality of the highest larvae of O. rhinoceros to Trichoderma sp fungus with a dose of 20 gr (96.67%) with the application method spread on the larvae. The highest amount of conidial density is found in Trichoderma sp fungi with doses of 60 gx 100-1 ml of aqua pro injection of 7.25 x 106 conidia/ml. Germination level of conidia mushroom highest in Trichoderma sp fungi with dose 60 gr x 100-1 ml aqua pro injection that is as much as 91%.