The prospects of cultivating Oyster mushroom (Pleurotus columbinus− P8) on date palm leaves with a combination of different agricultural waste, the supplementary material wheat bran, corn meal and gypsum were used at the rate of 5% of substrate dry weight in all treatments. Data were recorded from spawn running, pinhead formation, and growth of fruiting body, total number of fruiting bodies, yield and biological efficiency. Results revealed that treatment T4 (date palm leaves 25%+ wheat straw and cotton waste 75%) took minimum days for spawn running, pinhead formation, comparative growth of fruiting bodies and produce a maximum number of fruiting bodies. While treatment T1 (date palm leaves 100%) gave maximum days for spawn running, pinhead formation, comparative growth of fruiting bodies and produce a minimum number of fruiting bodies. The yield production and biological efficiency of Oyster mushroom was found to be higher in treatment T4,(date palm leaves 25%+ 75%(wheat straw+ cotton waste) gave 403 g and 80.6% respectively. While yield production and biological efficiency oyster mushroom was found lowest in treatment T1 (date palm leaves= 100% gave (185.75) g and (37.15)% respectively. Therefore, the technology of mushroom agriculture enables us to acquire substrate materials at very low cost and further led to conserve our environment through efficient bioconversion of wastes with sustainable food security.