Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system
Journal of virology, 2015•Am Soc Microbiol
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of
infectious diseases and treatment of cancer since its use for the eradication of smallpox.
However, the current method of editing the VACV genome is not efficient. Here, we
demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly
and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs
(gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
infectious diseases and treatment of cancer since its use for the eradication of smallpox.
However, the current method of editing the VACV genome is not efficient. Here, we
demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly
and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs
(gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
Abstract
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
American Society for Microbiology以上显示的是最相近的搜索结果。 查看全部搜索结果