The present study describes a new approach for the investigation of electrocatalytic oxidation of sulphide using a pencil graphite electrode modified with hematoxylin (PGE/HMT). Adsorption procedure was used for the preparation of the modified electrodes. It was observed that PGE/HMT showed a significant electrocatalytic activity toward sulphide oxidation. Cyclic voltammetric studies showed that the peak potential of sulphide shifted from +480mV at bare PGE to +220mV at PGE/HMT. The electrocatalytic currents obtained from amperometric measurements at +200mV versus Ag/AgCl/KCl_sat and at pH 8.0 BR buffer solution containing 0.1M KCl were linearly related to the concentration of sulphide. The calibration graph consisted of a linear segment of 1–200μM with a detection limit of 0.4μM (based on 3s_b). The proposed method was successfully applied to the determination of sulphide in waste waters and was compared with the spectrophotometric method.