Gold nanoelectrode ensembles (NEEs) have been prepared by using an electroless plating
method to deposit disk-shaped Au electrode elements (diameter= 38 nm) within the pores of
a microporous polycarbonate template membrane. The electrochemistry of three electron-
transfer mediators used for biosensors based on reductase enzymes—two phenothiazines
(Azure A and B) and methylviologen—were investigated at these NEEs. As has been
observed previously, detection limits obtained at the NEE are lower than corresponding …