Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography
JV Carratalá, J Atienza-Garriga, H López-Laguna… - Microbial cell …, 2023 - Springer
Background Recombinant proteins cover a wide range of biomedical, biotechnological, and
industrial needs. Although there are diverse available protocols for their purification from cell
extracts or from culture media, many proteins of interest such as those containing cationic
domains are difficult to purify, a fact that results in low yields of the final functional product.
Unfortunately, this issue prevents the further development and industrial or clinical
application of these otherwise interesting products. Results Aiming at improving the …
industrial needs. Although there are diverse available protocols for their purification from cell
extracts or from culture media, many proteins of interest such as those containing cationic
domains are difficult to purify, a fact that results in low yields of the final functional product.
Unfortunately, this issue prevents the further development and industrial or clinical
application of these otherwise interesting products. Results Aiming at improving the …
Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography
J Vicente Carratalá, J Atienza Garriga… - 2023 - ri.conicet.gov.ar
Background Recombinant proteins cover a wide range of biomedical, biotechnological, and
industrial needs. Although there are diverse available protocols for their purification from cell
extracts or from culture media, many pro‑teins of interest such as those containing cationic
domains are difficult to purify, a fat that results in low yields of thefinal functional product.
Unfortunately, this issue prevents the further development and industrial or clinical applica‑
tion of these otherwise interesting products. Results Aiming at improving the purification of …
industrial needs. Although there are diverse available protocols for their purification from cell
extracts or from culture media, many pro‑teins of interest such as those containing cationic
domains are difficult to purify, a fat that results in low yields of thefinal functional product.
Unfortunately, this issue prevents the further development and industrial or clinical applica‑
tion of these otherwise interesting products. Results Aiming at improving the purification of …
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