Recent studies in streams and ponds have demonstrated that the distribution and biomass
of aquatic organisms can be estimated by detection and quantification of environmental
DNA (eDNA). In more open systems such as seas, it is not evident whether eDNA can
represent the distribution and biomass of aquatic organisms because various environmental
factors (eg, water flow) are expected to affect eDNA distribution and concentration. To test
the relationships between the distribution of fish and eDNA, we conducted a grid survey in …

Fig 2. Spatial approximation of jack mackerel eDNA concentration. Based on CytB gene
copy number in a 2 μL template DNA solution at the 47 sampling station, spatial variation of
jack mackerel eDNA in west Maizuru Bay was estimated by approximation. The level of the
approximate eDNA concentration is indicated by colors between red (relatively high
concentration) and blue (low concentration). White areas suggest that the concentration
approximated using a regularized spline is 0. Spatial approximation was performed using a …