Equalisation of archival microscopic images from immunohistochemically stained tissue sections

U Neuman, A Korzyńska, C Lopez, M Lejeune… - Biocybernetics and …, 2013 - Elsevier
A method of image equalisation that reduces non-uniformity of light distribution caused by
optical devices and dust on camera sensors is presented. The method explores non-
uniformity which occurs in archival images captured by a typical optical set which consists of
a light microscope and a digital camera. A sufficient number of images with low density of
foreground objects has been used to extract a global map of non-uniformity of the particular
microscope and camera. The proposed method consists of two steps:–(1) extraction of the …
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