The purpose of this paper is to establish the current state of comfort and accessibility in public transportation by railway and how they interfere with the choices for transportation of the passengers and they’re well-being when using this services. This study is based on surveys made across Europe by various companies specialized in this field and on field data gathered in the past 3 years studying the behaviour of the passengers and time tables on various train routes in Romania. The research is focused on elderly people, people with disabilities and in some extent on normal travellers. The results of this paper will be relevant in the future development and design, regarding ergonomics, accessibility and comfort, in the public transportation area. The users of the railway transportation services were asked questions regarding their comfort, safety, security and accessibility to this services.