We have developed an efficient method for the preparation and maintenance of primary cell
cultures isolated from adult Mnemiopsis leidyi, a lobate ctenophore. Our primary cell cultures
are derived from tissue explants or enzymatically dissociated cells, and maintained in a
complex undefined ctenophore mesogleal serum. These methods can be used to isolate,
maintain and visually monitor ctenophore cells to assess proliferation, cellular morphology
and cell differentiation in future studies. Exemplar cell types that can be easily isolated from …

… We have developed an efficient method for the preparation and maintenance of primary
cell cultures isolated from adult Mnemiopsis leidyi, a lobate ctenophore. Our … Our goal
was to develop an easy and reliable method for generating primary cell cultures that can be
utilized to isolate representative cell types from the lobate ctenophore Mnemiopsis leidyi.
The protocols presented here will aid in the in vitro isolation and characterization of distinct
cell types associated with this enigmatic group of metazoans. …