Establishment of a reference single-cell RNA sequencing dataset for human pancreatic adenocarcinoma

R Chijimatsu, S Kobayashi, Y Takeda, M Kitakaze… - IScience, 2022 - cell.com
Single-cell RNA sequencing (scRNAseq) has been used to … microenvironment of pancreatic
ductal adenocarcinoma (PDAC). … Here, we built a single cell atlas of PDAC from six datasets

… -related prognostic signature predicts prognosis and immunotherapy response in pancreatic adenocarcinoma based on single-cell and bulk RNA-sequencing

Y Chen, Q Deng, H Chen, J Yang, Z Chen, J Li, Z Fu - Scientific Reports, 2023 - nature.com
… marker genes identified in single-cell RNA-sequencing data. … analysis resulted in the
development of a novel prognostic … PACA-AU dataset, which included RNA-seq data and clinical …

[HTML][HTML] Single-cell RNA sequencing to characterize the response of pancreatic cancer to anti-PD-1 immunotherapy

J Zhou, Y Jiang, Y Huang, Q Wang, JT Kaifi… - Translational …, 2022 - Elsevier
Pancreatic cancer (PaC) is resistant to immune checkpoint … In this study, we have established
four orthotopic PaC … , the single-cell datasets for Panc02- and Panc02-H7-tumors were …

Immune profiling and prognostic model of pancreatic cancer using quantitative pathology and single-cell RNA sequencing

K Chen, Q Wang, X Liu, X Tian, A Dong… - Journal of Translational …, 2023 - Springer
establish a new prognostic model. We also conducted single-cell RNA sequencing (scRNA-seq) …
The scRNA-seq dataset for the PDAC and adjacent noncancerous specimens has been …

… of pancreatic ductal adenocarcinoma diagnosis using a blood immune cell biomarker panel developed from bulk mRNA sequencing and single-cell RNA-sequencing

SI Jang, HK Lee, EJ Chang, S Kim, SY Kim… - Cancer Immunology …, 2023 - Springer
human PBMC scRNA-seq dataset as a single-cell reference. … pancreatic cancer from
non-pancreatic cancer. Therefore, combinations of these three biomarkers were used to establish

Single-cell sequencing and establishment of an 8-gene prognostic model for pancreatic cancer patients

X Yu, Q Zhang, S Zhang, Y He, W Guo - Frontiers in Oncology, 2022 - frontiersin.org
… significantly improved RNA sequencing (RNA-seq) methods … , preserved pancreatic cancer
sample data of six datasets (GEO… pancreatic cancer at different stages using single-cell RNA-…

Single-cell RNA-seq reveals the genesis and heterogeneity of tumor microenvironment in pancreatic undifferentiated carcinoma with osteoclast-like giant-cells

X Wang, J Miao, S Wang, R Shen, S Zhang, Y Tian… - Molecular Cancer, 2022 - Springer
… of pancreatic cancer for the marker genes in our work [34]. … TCGA-PAAD and GTEx-pancreas
dataset on GEPIA website. It … pancreatic tumors have the potential to promote the formation

Single-cell RNA sequencing revealed subclonal heterogeneity and gene signatures of gemcitabine sensitivity in pancreatic cancer

Z Hou, J Lin, Y Ma, H Fang, Y Wu, Z Chen… - Frontiers in …, 2023 - frontiersin.org
… -resistant subclone and established a gemcitabine sensitivity … ) in The Cancer Genome Atlas
(TCGA) training dataset. The … /ml in preparation for single-cell library creation. Cell viability …

Occult polyclonality of preclinical pancreatic cancer models drives in vitro evolution

ME Monberg, H Geiger, JJ Lee, R Sharma… - Nature …, 2022 - nature.com
… Here we apply single-cell sequencing technologies to reveal … hybridization, and single-cell
RNA sequencing (scRNAseq). … in a panel of established and globally utilized PDAC cell lines (…

Combining single-cell and bulk RNA sequencing, NK cell marker genes reveal a prognostic and immune status in pancreatic ductal adenocarcinoma

Y Ouyang, R Shen, L Chu, C Fu, W Hu, H Huang… - Scientific Reports, 2024 - nature.com
… was validated by 6 GEO datasets and had an excellent evaluation. … in cancer and drive
cancer development 30 . Based on the importance of KRAS, TP53 and SCNA in pancreatic cancer