Present study aimed at estimating soil loss in Kherot watershed of Pratapgarh district, Rajasthan. Soil loss was estimated by using Revised Universal Soil Loss Equation (RUSLE) using Remote Sensing and Geographic Information System (GIS) techniques. GIS-based maps of five RUSLE factors (R, K, LS, C, P) were generated for the study area. Raster maps of the RUSLE factors were multiplied to estimate sediment yield on pixel basis. It was apparent from the study that in the year 2015, 164.0 ha area (72%) was under the very slight erosion class which covered major portion of the study area. Severe, very severe and extremely severe classes covered 19.60 ha area (8.63%). In the year 2017, 172.0 ha area (75.44%) was under the very slight erosion class which covered major portion of the study area. Sever, very severe and extremely severe classes covered 14.30 ha area (6.26%).