Evaluation of 2 '-modified oligonucleotides containing 2 '-deoxy gaps as antisense inhibitors of gene expression
BP Monia, EA Lesnik, C Gonzalez, WF Lima… - Journal of Biological …, 1993 - ASBMB
We have used a previously described 17-mer phosphorothioate (Monia, BP, Johnston, JF,
Ecker, DJ, Zounes, MA, Lima, WF, and Freier, SM (1992) J. Biol. Chem. 267, 19954-19962)
for structure-function analysis of 2'-sugar modifications including 2'-O-methyl, 2'-O-propyl, 2'-
O-pentyl, and 2'-fluoro. These modifications were analyzed for hybridization affinity to
complementary RNA and for antisense activity against the Ha-ras oncogene in cells using a
highly sensitive transactivation reporter gene system. Hybridization analysis demonstrated …
Ecker, DJ, Zounes, MA, Lima, WF, and Freier, SM (1992) J. Biol. Chem. 267, 19954-19962)
for structure-function analysis of 2'-sugar modifications including 2'-O-methyl, 2'-O-propyl, 2'-
O-pentyl, and 2'-fluoro. These modifications were analyzed for hybridization affinity to
complementary RNA and for antisense activity against the Ha-ras oncogene in cells using a
highly sensitive transactivation reporter gene system. Hybridization analysis demonstrated …
以上显示的是最相近的搜索结果。 查看全部搜索结果