[PDF][PDF] Evaluation of Polymerase Chain Reaction for the detection of Vibrio cholerae in Contaminants

M Maheshwari, N Krishnaiah, D Ramana - Ann. Biol. Res, 2011 - core.ac.uk
ABSTRACT A total of 245 samples (35 each of water, fish, crab, shrimp, meat, milk and
clinical stool samples) collected from various sources were subjected to Polymerase Chain
Reaction (PCR) and cultural methods for the presence of Vibrio cholerae. Eighty samples
(19 water, 16 fish, 20 crab, 6 shrimp, 4 meat, 3 milk, and 12 clinical stool samples) were
positive by PCR targeting ompW gene, whereas only 59 samples (12 water, 13 fish, 16 crab,
5 shrimp, 3 meat, 2 milk and 8 clinical stool samples) were positive by cultural methods …

Evaluation of Polymerase Chain Reaction for the detection of Vibrio cholerae in contaminants.

MM Mani Maheshwari, N Krishnaiah, DBV Ramana - 2011 - cabidigitallibrary.org
A total of 245 samples (35 each of water, fish, crab, shrimp, meat, milk and clinical stool
samples) collected from various sources were subjected to Polymerase Chain Reaction
(PCR) and cultural methods for the presence of Vibrio cholerae. Eighty samples (19 water,
16 fish, 20 crab, 6 shrimp, 4 meat, 3 milk, and 12 clinical stool samples) were positive by
PCR targeting ompW gene, whereas only 59 samples (12 water, 13 fish, 16 crab, 5 shrimp, 3
meat, 2 milk and 8 clinical stool samples) were positive by cultural methods. Enrichment with …
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