Present study was designed to evaluate the physicochemical, biological and humic substances status of the Bangshi, Dhaleshwari and Padma rivers which are located at the central part of Bangladseh. These rivers were selected as study area due to the presence of different effluent discharging industries in surrounding areas as well as for their ecological and local economy contributions. Dissolved Oxygen (DO), pH, Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Dissolved Solid (TDS), turbidity, Electrical Conductivity (EC), Total Coliform and Total Organic Carbon (TOC) were determined to evaluate the physicochemical and biological water quality and compared with Department of Environment (DoE), Government of Bangladesh, surface water quality standard. Analyses of theses parameters indicates high pollution load in the rivers although samples were collected in the rainy season. Dissolves organic matter (DOM) fluorescence was used to determine the humic substance status of the rivers. Humic substances or organic matter pollution was evident by the presence of protein like substances, humic and fulvic acids in the samples. It is high time to design effective seasonal river water quality monitoring program and take necessary measures to protect environment and ensure sustainable human development.