Evidence for a triplex DNA conformation at the bcl-2 major breakpoint region of the t (14; 18) translocation
The most common chromosomal translocation in cancer, t (14; 18), occurs at the bcl-2 major
breakpoint region (Mbr) in follicular lymphomas. The 150-bp bcl-2 Mbr, which contains three
breakage hotspots (peaks), has a single-stranded character and, hence, a non-B DNA
conformation both in vivo and in vitro. Here, we use gel assays and electron microscopy to
show that a triplex-specific antibody binds to the bcl-2 Mbr in vitro. Bisulfite reactivity shows
that the non-B DNA structure is favored by, but not dependent upon, supercoiling and …
breakpoint region (Mbr) in follicular lymphomas. The 150-bp bcl-2 Mbr, which contains three
breakage hotspots (peaks), has a single-stranded character and, hence, a non-B DNA
conformation both in vivo and in vitro. Here, we use gel assays and electron microscopy to
show that a triplex-specific antibody binds to the bcl-2 Mbr in vitro. Bisulfite reactivity shows
that the non-B DNA structure is favored by, but not dependent upon, supercoiling and …
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