Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation and exocytosis in heterotroph cell cultures of Chenopodium rubrum
A Chaidee, C Wongchai, W Pfeiffer - Journal of plant physiology, 2008 - Elsevier
We investigated the response of extracellular phosphatase to heat shock in heterotrophic
Chenopodium rubrum L. cell cultures. Surprisingly, in contrast to the generally used acid
phosphatase, an extracellular alkaline phosphatase showed the most sensitive response.
This phosphatase was characterized as a marker for cellular stimulation by its high
correlations with induced changes of extracellular pH: 10μM nigericin (correlation coefficient
r= 0.91), 100μM salicylic acid (r= 0.84), heat shock 5min 37° C (r= 0.79), and heat shock …
Chenopodium rubrum L. cell cultures. Surprisingly, in contrast to the generally used acid
phosphatase, an extracellular alkaline phosphatase showed the most sensitive response.
This phosphatase was characterized as a marker for cellular stimulation by its high
correlations with induced changes of extracellular pH: 10μM nigericin (correlation coefficient
r= 0.91), 100μM salicylic acid (r= 0.84), heat shock 5min 37° C (r= 0.79), and heat shock …
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