Fish oil is one of the most valuable marine products that has been used as unrefined for animal feed, poultry and aquaculture, and can be used by human due to existence of the Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA). In this study, oil extraction was studied with two methods of supercritical fluid (SFE) and wet press (WP) from by-products of canned tuna factory from two aspects of yield and product quality, focusing on human consumption. For this purpose, the ratio of the obtained oil from the raw material as well as the lipid peroxidation indices were evaluated. The product yield rate did not show significant different in either of the two methods. Some volatile compounds such as aldehydes were observed only in the WP method, and the number of found alkanes in this method was higher than that of SFE. In contrast, citric acid as non-lipid organic acid was found only in SFE treatment, and dimethyl amine (DMA) was found to be the main cause of the specific smell of fish in each of the two treatments. Therefore, the results of this study indicate the potential of tuna by-products for extraction of fish oil for human consumption and the SFE method for extracting it.