The skin losses can occur due to acute trauma, resection of cutaneous malignancies, donor site harvesting, chronic wounds or even surgical interventions. The full thickness injuries are characterized by the complete destruction of epithelial regenerative tissues. Currently, autologous skin grafting is the treatment of choice for full thickness skin injuries. In this process donor site also heal with some scarring and may be very painful. The large wound that can not be corrected by conventional surgical procedures requires substitute of missing tissue to keep the wound free of infection, to reduce pain and ensure early wound healing [1]. In the cases of extensive injuries, donor sites are extremely limited and in such cases alternative life saving approaches are required. This may include the use of bioengineered skin substitutes. The dermal substitutes effectively close the wound and help to guide cells during granulation tissue formation, fibroblast proliferation, angiogenesis and epithelialization [1]. Tissue engineering is a promising strategy for tissue regeneration. The concept of tissue engineering allows in-vitro expansion of isolated cells using cell culture techniques and their transplantations for organ regeneration. A tissue engineered construct is produced by culturing the required cell types on a biocompatible scaffold or extracellular matrix (ECM)[2]. The scaffolds have been developed from synthetic biodegradable polymers, natural polymers and natural matrices derived from decellularised tissue [3]. The natural bio-scaffold have advantages over synthetic material that it mimic natural ECM structure and composition, simulate natural stimulatory effects of ECM on cells and allows the incorporation of growth factors and other matrix proteins to further enhance cell functions. The scaffold allows cell invasion, their proliferation and secretion of own extra cellular matrix for longer duration leading to a complete and natural tissue replacement [4].

In skin tissue engineering the ideal matrix/scaffold should be able to provide the right biological and physiological environment to ensure homologous distribution of cells and ECM. It should also provide the right size and morphology of the neo-tissue required. It must not induce a toxic or immune response or result in extensive inflammation. It should have low level of disease risk, be slowly biodegradable, support the reconstruction of normal tissue and have similar mechanical and physical properties to the skin that it replaces. The matrix also supplies growth factors and cell signaling molecules that are required by the cells to self organize in to a 3-D functional tissue [5]. Dermal substitute scaffolds promote