Ergosterol is a principle sterol and an economically important metabolite produced by the Saccharomyces cerevisiae. This compound is a pro vitamin or a precursor to vitamin D2.. Ergosterol plays an important role in physiological function and in pharmaceutical clinical trials. It could be used in the treatment of fungal infection and rickets (disease caused by vitamin D deficiency). This project is aimed to exploit the potential of Saccharomyces cerevisiae to produce ergosterol from corn straw hydrolysate and soybean meal liquor as an inexpensive carbon source. Yeast strains were isolated from grape juice and characterized. Corn straw was pretreated by the process of acid hydrolysis and soybean meal liquor was prepared by boiling and filtration followed by the fermentation on malt wort medium and YEPD medium using Saccharomyces cerevisiae as the production strain. Ergosterol was extracted using Petroleum ether as solvent. The produced ergosterol was analyzed using Fourier Transform Infra-Red Spectroscopy (FTIR) and Gas chromatography