Filtration of Commercial Extenders Prior to Refreezing Stallion Sperm for ICSI
GD Catandi, RA Gonzalez-Castro, JM Trentin… - Journal of Equine …, 2018 - Elsevier
2. Materials and Methods A single ejaculate from each of twelve stallions was frozen in EZ
Freezin® Cryomax LE (Animal Reproduction Systems, ARS, Chino, CA). Four 0.5-mL straws
of each of the twelve sperm samples were thawed at 37 C for 30 sec and pooled. Prior to
refreezing, thawed samples were divided and diluted in three commercial extenders that
were not filtered or filtered: 1) BOT (Botucrio®, Betlabs, Lexington, KY), 2) CRYO (EZ
Freezin® Cryomax™ MFR5, ARS), and 3) MFR5 (EZ Freezin® MFR5, ARS). For filtering …
Freezin® Cryomax LE (Animal Reproduction Systems, ARS, Chino, CA). Four 0.5-mL straws
of each of the twelve sperm samples were thawed at 37 C for 30 sec and pooled. Prior to
refreezing, thawed samples were divided and diluted in three commercial extenders that
were not filtered or filtered: 1) BOT (Botucrio®, Betlabs, Lexington, KY), 2) CRYO (EZ
Freezin® Cryomax™ MFR5, ARS), and 3) MFR5 (EZ Freezin® MFR5, ARS). For filtering …
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